Molecular characterization of Salmonella Typhimurium and Salmonella Enteritidis by plasmid analysis and pulsed-field gel electrophoresis

被引：35

作者：

Aktas, Zerrin ^{[1
]}

Day, Martin ^{[2
]}

Kayacan, Cigdem Bal ^{[1
]}

Direnc, Sukufe ^{[3
]}

Threlfall, E. John ^{[2
]}

机构：

[1] Istanbul Univ, Istanbul Fac Med, Dept Microbiol & Clin Microbiol, Istanbul, Turkey

[2] Hlth Protect Agcy Ctr Infect, Lab Enter Pathogens, London NW9 5EQ, England

[3] Istanbul Univ, Cerrahpasa Fac Med, Dept Pediat, Microbiol Lab, Istanbul, Turkey

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2007年 / 30卷 / 06期

关键词：

Salmonella Typhimurium DT 104; ACSSuT; Salmonella Enterifidis; plasmid profile analysis;

D O I：

10.1016/j.ijantimicag.2007.06.034

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Forty-one paediatric isolates of Salmonella spp. from Cerrahpasa Faculty of Medicine, Istanbul, Turkey, between 2001 and 2004 were examined for susceptibility to various antibiotics and presence of antibiotic resistance genes. Pulsed-field gel electrophoresis and plasmid profiling were used to determine possible genetic relationships among Salmonella enterica subsp. enterica clinical isolates. Plasmids from resistant strains were not transferred by conjugation to recipient Escherichia coli cells. Pulsed-field gel electrophoresis and restriction enzyme digestion analysis of DNA revealed that multidrug-resistant isolates belonged to the same clonal group, characterized by ACSSuT resistotype. Isolates of R-type ACSSuT were positive for the intI gene and possessed a single plasmid of 60 MDa. (C) 2007 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：541 / 545

页数：5

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