Development and evaluation of antisera for detection of the O,O-diethyl phosphorothionate and phosphorothionothiolate organophosphorus pesticides by immunoassay

被引：50

作者：

Johnson, JC ^{[1
]}

Van Emon, JM ^{[1
]}

Pullman, DR ^{[1
]}

Keeper, KR ^{[1
]}

机构：

[1] US EPA, Human Exposure Res Branch, Human Exposure & Atmospher Sci Div, Las Vegas, NV 89119 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1998年 / 46卷 / 08期

关键词：

organophosphorus pesticides; immunoassay; class-specific detection;

D O I：

10.1021/jf970191y

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

An immunochemical approach for class detection of organophosphorus pesticides was investigated. Synthesis of O,O-diethyl phosphorothionate haptens and their use in the generation of polyclonal antisera are described. An indirect inhibition format ELISA coupled with an automated screening procedure was used to characterize the dose-response of the resulting antisera for 17 representative O,O-diethyl and O,O-dimethyl phosphorothionates/thionothiolates and phosphates.

引用

页码：3116 / 3123

页数：8

共 22 条

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