Identification and characterization of an ecto-ATPase activity in rat Sertoli cells

被引：24

作者：

Barbacci, E

Filippini, A

DeCesaris, P

Ziparo, E

机构：

[1] UNIV ROMA LA SAPIENZA,SCH MED,INST HISTOL & GEN EMBRYOL,ROME,ITALY

[2] UNIV LAQUILA,DEPT EXPTL MED,I-67100 LAQUILA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 222卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.0734

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A membrane associated extracellular ATPase (ecto) has been identified on rat Sertoli cells. Sertoli cell ecto-ATPase demonstrated a Km for ATP of 114 mu M and a Vmax of 1.79 mu mol/min/2 x 10(5) cells and was activated by either Mg2+ or Ca2+. This ecto-ATPase hydrolyzes other nucleoside triphosphates, but is inactive with ADP. The effects of some possible inhibitors of ectonucleotidases on the breakdown of extracellular ATP by Sertoli cells were also investigated. (C) 1996 Academic Press, Inc.

引用

收藏

页码：273 / 279

页数：7

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