The nickel-containing enzyme urease is an essential colonization factor of the human gastric pathogen Helicobacter pylori which enables the bacteria to survive the low-pH conditions of the stomach. Transcription of the urease genes is positively controlled in response to increasing concentrations of nickel ions and acidic pH. Here we demonstrate that acid-induced transcription of the urease genes is mediated directly by the ArsRS two-component system. Footprint analyses identify binding sites of the phosphorylated ArsR response regulator within the ureA and ureI promoters. Furthermore, deletion of a distal upstream ArsR binding site of the ureA promoter demonstrates its role in acid-dependent activation of the promoter. In addition, acid-induced transcription of the ureA gene is unaltered in a nikR mutant, providing evidence that pH-responsive regulation and nickel-responsive regulation of the ureA promoter are mediated by independent mechanisms involving the ArsR response regulator and the NikR protein.
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Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Bury-Moné, S
Thiberge, JM
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Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Thiberge, JM
Contreras, M
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Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Contreras, M
Maitournam, A
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Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Maitournam, A
Labigne, A
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Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Labigne, A
De Reuse, H
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Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
机构:Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France
Contreras, M
Thiberge, JM
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h-index: 0
机构:Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France
Thiberge, JM
Mandrand-Berthelot, MA
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机构:Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France
Mandrand-Berthelot, MA
Labigne, A
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Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France
机构:
Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Bury-Moné, S
Thiberge, JM
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h-index: 0
机构:
Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Thiberge, JM
Contreras, M
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h-index: 0
机构:
Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Contreras, M
Maitournam, A
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h-index: 0
机构:
Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Maitournam, A
Labigne, A
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h-index: 0
机构:
Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
Labigne, A
De Reuse, H
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h-index: 0
机构:
Inst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueusees, F-75724 Paris 15, France
机构:Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France
Contreras, M
Thiberge, JM
论文数: 0引用数: 0
h-index: 0
机构:Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France
Thiberge, JM
Mandrand-Berthelot, MA
论文数: 0引用数: 0
h-index: 0
机构:Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France
Mandrand-Berthelot, MA
Labigne, A
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, FranceInst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France