Acid-induced activation of the urease promoters is mediated directly by the ArsRS two-component system of Helicobacter pylori

被引:81
作者
Pflock, M
Kennard, S
Delany, I
Scarlato, V
Beier, D [1 ]
机构
[1] Univ Wurzburg, Theodor Boveri Inst Biowissenschaft, Lehrstuhl Mikrobiol, D-97074 Wurzburg, Germany
[2] Chiron Vaccines, Mol Immunol Unit, I-53100 Siena, Italy
关键词
D O I
10.1128/IAI.73.10.6437-6445.2005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The nickel-containing enzyme urease is an essential colonization factor of the human gastric pathogen Helicobacter pylori which enables the bacteria to survive the low-pH conditions of the stomach. Transcription of the urease genes is positively controlled in response to increasing concentrations of nickel ions and acidic pH. Here we demonstrate that acid-induced transcription of the urease genes is mediated directly by the ArsRS two-component system. Footprint analyses identify binding sites of the phosphorylated ArsR response regulator within the ureA and ureI promoters. Furthermore, deletion of a distal upstream ArsR binding site of the ureA promoter demonstrates its role in acid-dependent activation of the promoter. In addition, acid-induced transcription of the ureA gene is unaltered in a nikR mutant, providing evidence that pH-responsive regulation and nickel-responsive regulation of the ureA promoter are mediated by independent mechanisms involving the ArsR response regulator and the NikR protein.
引用
收藏
页码:6437 / 6445
页数:9
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