Crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the β1,4-galactosyltransferase-1

被引:166
作者
Ramakrishnan, B
Qasba, PK [1 ]
机构
[1] NCI, CCR, Struct Glycobiol Sect, Frederick, MD 21702 USA
[2] NCI, Intramural Res Support Program, SAIC, Lab Expt & Computat Biol,CCR, Frederick, MD 21702 USA
关键词
lactose synthase; crystal structure; substrate binding; conformational changes; modulation;
D O I
10.1006/jmbi.2001.4757
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The lactose synthase (LS) enzyme is a 1:1 complex of a catalytic component, beta1,4-galactosyltransferse (beta 4Gal-T1) and a regulatory component, a-lactalbumin (LA), a mammary gland-specific protein. LA promotes the binding of glucose (Glc) to beta 4Gal-T1, thereby altering its sugar acceptor specificity from N-acetylglucosamine (GlcNAc) to glucose, which enables LS to synthesize lactose, the major carbohydrate component of milk. The crystal structures of LS bound with various substrates were solved at 2 Angstrom resolution. These structures reveal that upon substrate binding to beta 4Gal-T1, a large conformational change occurs in the region comprising residues 345 to 365. This repositions His347 in such a way that it can participate in the coordination of a metal ion, and creates a sugar and LA-binding site. At the sugar-acceptor binding site, a hydrophobic N-acetyl group-binding pocket is found, formed by residues Arg359, Phe360 and Ile363. In the Glc-bound structure, this hydrophobic pocket is absent. For the binding of Glc to LS, a reorientation of the Arg359 sidechain occurs, which blocks the hydrophobic pocket and maximizes the interactions with the Glc molecule. Thus, the role of LA is to hold Glc by hydrogen bonding with the O-1 hydroxyl group in the acceptor-binding site on beta 4Gal-T1, while the N-acetyl group-binding pocket in beta 4Gal-T1 adjusts to maximize the interactions with the Glc molecule. This study provides details of a structural basis for the partially ordered kinetic mechanism proposed for lactose synthase.
引用
收藏
页码:205 / 218
页数:14
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