Large plasmids of avian Escherichia coli isolates

被引:26
作者
Doetkott, DM [1 ]
Nolan, LK [1 ]
Giddings, CW [1 ]
Berryhill, DL [1 ]
机构
[1] N DAKOTA STATE UNIV, DEPT VET & MICROBIOL SCI, FARGO, ND 58105 USA
关键词
avian Escherichia coli; analysis of plasmids; avian colibacillosis;
D O I
10.2307/1592319
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The plasmid DNA of 30 Escherichia coli isolates from chickens was extracted and examined using techniques designed to isolate large plasmids. This plasmid DNA was examined for the presence of certain known virulence-related genes including cvaC, traT, and some aerobactin-related sequences. Seventeen of the 30 isolates contained from one to four plasmids greater than 50 kb in size. Eleven of these 17 strains possessed plasmids greater than 100 kb in size. Therefore, E. coli isolates of chickens frequently contain large plasmids, and many of these plasmids are likely to contain virulence-related sequences.
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收藏
页码:927 / 930
页数:4
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