Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil-miscible KOH/propanol/methanol medium

被引:11
作者
Mendonça, CRB
Bica, CID
Piatnicki, CMS
Simó-Alfonso, EF
Ramis-Ramos, G
机构
[1] Univ Valencia, Dept Quim Anal, E-46100 Burjassot, Spain
[2] Univ Fed Rio Grande Sul, Inst Quim, Porto Alegre, RS, Brazil
关键词
biophenols; hydroxyaromatic compounds; synthetic antioxidants; tocopherols; vegetable oils;
D O I
10.1002/elps.200410394
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The separation of hydroxyaromatic compounds in vegetable oils, including synthetic antioxidants (3-tert-butyl-4-hydroxyanisol and 2,6-di-tert-butyl-4-hydroxytoluene), E-vitamers and other natural oil components, by nonaqueous capillary electrophoresis in an oil-miscible background electrolyte (BGE) was investigated. The BGE contained 40 mm KOH in a methanol/1-propanol (PrOH) mixture (15:85 v/v). The oil samples were 1:1 diluted with PrOH and directly injected in the capillary. Under negative polarity (cathode at the injection end), the anionic solutes moved faster than the electroosmotic flow, being well-resolved among them and from the triacylglycerols. Using virgin palm, extra virgin olive, wheat germ, virgin soybean and other oils, the capability of the procedure to quickly yield a characteristic profile of the biophenols present in the sample was demonstrated. The alpha-, (beta + gamma)-(as unresolved pair) and delta-tocopherols of a soybean oil sample were quantified.
引用
收藏
页码:3307 / 3314
页数:8
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