The human thymic microenvironment during organ culture

被引:13
作者
Markert, ML
Watson, TJ
Kaplan, I
Hale, LP
Haynes, BF
机构
[1] DUKE UNIV, MED CTR, DEPT IMMUNOL, DURHAM, NC USA
[2] DUKE UNIV, MED CTR, DEPT PATHOL, DURHAM, NC 27710 USA
[3] DUKE UNIV, MED CTR, DEPT MED, DIV RHEUMATOL ALLERGY & CLIN IMMUNOL, DURHAM, NC 27710 USA
来源
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1997年 / 82卷 / 01期
关键词
D O I
10.1006/clin.1996.4266
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Cultured human thymic tissue has been transplanted into many patients with T cell dysfunction; however, little is known about the effect of in vitro culture on thymic tissue. Human postnatal thymic organ cultures were established in vitro to study the growth potential of the thymic epithelium and the expression of intracellular and surface antigens with time in culture. Marked depletion of bone marrow-derived cells was observed within 3 weeks of initiation of organ cultures although some viable CD3+ cells could still be detected. Thymic epithelial cells in in vitro explants continued to express MHC class I and class II antigens as well as cytokeratins. Thymic epithelial cells within cultured thymic organ slices maintained their postnatal growth potential, in that cytokeratin-positive epithelial monolayers could be established in vitro from these thymic slices up to 12 weeks after initiation of organ culture. Thus, thymic explants remained viable in culture and could potentially be used to reconstitute immunity in T cell deficient patients. (C) 1997 Academic Press, Inc.
引用
收藏
页码:26 / 36
页数:11
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