Simultaneous on-line extraction and analysis of sirolimus (rapamycin) and ciclosporin in blood by liquid chromatography electrospray mass spectrometry

被引:47
作者
Kirchner, GI
Vidal, C
Jacobsen, W
Franzke, A
Hallensleben, K
Christians, U
Sewing, KF
机构
[1] Med Hsch Hannover, Inst Allgemeine Pharmakol, D-30625 Hannover, Germany
[2] Med Hsch Hannover, Inst Hamatol & Onkol, D-30625 Hannover, Germany
[3] Univ Calif San Francisco, Dept Biopharmaceut Sci, Sch Pharm, San Francisco, CA 94143 USA
来源
JOURNAL OF CHROMATOGRAPHY B | 1999年 / 721卷 / 02期
关键词
sirolimus; rapamycin; ciclosporin;
D O I
10.1016/S0378-4347(98)00463-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We developed a sensitive and specific semi-automated liquid chromatography-electrospray mass spectrometric (HPLC-ESI-MS) assay for the simultaneous quantification of sirolimus and ciclosporin in blood. Following a simple protein precipitation step, the supernatants were injected into the HPLC system and extracted on-line. After column switching, the analytes were backflushed from the extraction column onto the analytical narrow-bore column and eluted into the ESI-MS system. The assay was linear from 0.4 to 100 mu g/l sirolimus and from 2 to 1500 mu g/l ciclosporin. The mean recoveries of sirolimus and ciclosporin were 98 and 96%, respectively. The mean interday precision/accuracy was 8.6%/-4.8% for sirolimus and 9.3%/-2.9% for ciclosporin. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:285 / 294
页数:10
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