De novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics

被引：42

作者：

Kalb, Suzanne R. ^{[1
]}

Baudys, Jakub ^{[1
]}

Rees, Jon C. ^{[1
]}

Smith, Theresa J. ^{[2
]}

Smith, Leonard A. ^{[3
]}

Helma, Charles H. ^{[4
]}

Hill, Karen ^{[4
]}

Kull, Skadi ^{[5
]}

Kirchner, Sebastian ^{[5
]}

Dorner, Martin B. ^{[5
]}

Dorner, Brigitte G. ^{[5
]}

Pirkle, James L. ^{[1
]}

Barr, John R. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Natl Ctr Environm Hlth, Div Sci Lab, Atlanta, GA 30341 USA

[2] USAMRIID, Ft Detrick, MD 21702 USA

[3] MRMC, Off Chief Scientist, Ft Detrick, MD 21702 USA

[4] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA

[5] Robert Koch Inst, Ctr Biol Secur, Microbial Toxins ZBS3, D-13353 Berlin, Germany

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2012年 / 403卷 / 01期

关键词：

Botulinum neurotoxin; Botulism; Mass spectrometry; Proteomics; CLOSTRIDIUM-BOTULINUM; INFANT BOTULISM; JAPAN; DIFFERENTIATION; DIVERSITY; SEROTYPE;

D O I：

10.1007/s00216-012-5767-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Botulinum neurotoxins (BoNTs) cause the disease botulism, which can be lethal if untreated. There are seven known serotypes of BoNT, A-G, defined by their response to antisera. Many serotypes are distinguished into differing subtypes based on amino acid sequence, and many subtypes are further differentiated into toxin variants. Previous work in our laboratory described the use of a proteomics approach to distinguish subtype BoNT/A1 from BoNT/A2 where BoNT identities were confirmed after searching data against a database containing protein sequences of all known BoNT/A subtypes. We now describe here a similar approach to differentiate subtypes BoNT/B1, /B2, /B3, /B4, and /B5. Additionally, to identify new subtypes or hitherto unpublished amino acid substitutions, we created an amino acid substitution database covering every possible amino acid change. We used this database to differentiate multiple toxin variants within subtypes of BoNT/B1 and B2. More importantly, with our amino acid substitution database, we were able to identify a novel BoNT/B subtype, designated here as BoNT/B7. These techniques allow for subtype and strain level identification of both known and unknown BoNT/B rapidly with no DNA required. '

引用

页码：215 / 226

页数：12

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