Use of Rab GTPases to study lipid trafficking in normal and sphingolipid storage disease fibroblasts

被引：4

作者：

Choudhury, A ^{[1
]}

Marks, DL

Pagano, RE

机构：

[1] Mayo Clin, Coll Med, Dept Biochem & Mol Biol, Thorac Dis Res Unit, Rochester, MN 55905 USA

[2] Univ Basel, Biozentrum, Basel, Switzerland

来源：

GTPASES REGULATING MEMBRANE TARGETING AND FUSION | 2005年 / 403卷

关键词：

D O I：

10.1016/S0076-6879(05)03014-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We describe methods for studying lipid transport in normal and sphingolipid storage disease fibroblasts. These techniques include endocytic assays with fluorescent sphingolipid analogs, expression of dominant negative (DN) Rab GTPases, and methods of manipulating cholesterol levels in intact cells and isolated cell membranes. These methods should be useful in future studies of lipid trafficking in normal and disease cell types.

引用

页码：166 / 182

页数：17

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