Lipase production by solid state fermentation of olive cake and sugar cane bagasse

Olive oil cake (OOC) from Morocco and sugar cane bagasse (SCB) were used for lipase production using thermostable fungal cultures of Rhizomucor pusillus and Rhizopus rhizopodiformis. The maximum production of lipase by Rhizomucor pusillus and Rhizopus rhizopodiformis in solid state fermentation (SSF) using SCB, was 4.99 U/g DM equivalent to 1.73 U/ml and 2.67 U/g DM equivalent to 0.97 U/ml, respectively. However, the mixture of OOC and SCB, 50% each, increased the lipase activity as high as 79.6 U/g DM equivalent to 43.04 U/ml and 20.24 U/g DM equivalent to 10.83 U/ml obtained by Rhizopus rhizopodiformis and Rhizomucor pusillus, respectively. These data compare favourably with most of the activities reported for other Lipase hyperproducing microorganisms. (C) 1998 Published by Elsevier Science B.V. All rights reserved.