Low temperature induces the delivery of mature and immature CFTR to the plasma membrane

被引:37
作者
Rennolds, Jessica [1 ]
Boyaka, Prosper N. [1 ,2 ]
Bellis, Susan L. [3 ]
Cormet-Boyaka, Estelle [1 ]
机构
[1] 201 Davis Heart & Lung Res Inst, Div Pulm Crit Care & Sleep Med, Dept Internal Med, Columbus, OH 43210 USA
[2] Ohio State Univ, Dept Vet Biosci, Columbus, OH 43210 USA
[3] Univ Alabama, Dept Physiol & Biophys, Birmingham, AL 35294 USA
关键词
cystic fibrosis; CFTR; Delta F508; trafficking; biotinylation;
D O I
10.1016/j.bbrc.2007.12.065
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Deletion of phenylalanine 508 (Delta F508) is the most prevalent disease-causing mutation resulting in retention of the immature CFTR in the endoplasmic reticulum. The most common strategy to induce the delivery of Delta F508-CFTR to the surface of cells is by reducing the incubation temperature (approximate to 28 degrees C). Cell surface biotinylation of HEK293T cells grown at 37 degrees C for 48 h, confirmed the presence of mature wild-type CFTR, but not AF508-CFTR at the cell surface. On the other hand, cells incubated at 28 degrees C for 16 h showed both mature and immature Delta F508-CFTR at their surface. The trafficking of immature Delta F508-CFTR, but not mature Delta F508-CFTR, to the cell surface occurred at low temperature even upon addition of BFA, suggesting the involvement of a Golgi-independent pathway. These results suggest that low temperature induces the appearance of a mix population of mature and immature CFTR molecules at the plasma membrane through distinct pathways. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:1025 / 1029
页数:5
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