A sensitive assay of human blood platelet cyclic nucleotide phosphodiesterase activity by HPLC using fluorescence derivatization and its application to assessment of cyclic nucleotide phosphodiesterase inhibitors

被引：6

作者：

Ohba, Y ^{[1
]}

Soda, K ^{[1
]}

Zaitsu, K ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2001年 / 24卷 / 05期

关键词：

3'; 5'-cyclic nucleotide phosphodiesterase; cGMP; platelet human; derivatization fluorescence; HPLC;

D O I：

10.1248/bpb.24.567

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A selective and sensitive HPLC measurement of 3 ' ,5 ' -cyclic nucleotide phosphodiesterase (PDE) activity in human platelets using (3,4-dimethoxyphenyl)glyoxal (DMPG) as a fluorogenic reagent for guanine and its nucleosides and nucleotides is described. cGMP, a substrate for PDE, and GMP, which was produced by the enzyme reaction, are selectively converted by the reaction with DMPG to the fluorescent derivatives. The derivatives were separated by reversed-phase HPLC. Human platelet PDE activity was measured and the inhibitory effects of several compounds were investigated.

引用

页码：567 / 569

页数：3

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