Novel gel-based rapid test for non-instrumental detection of ochratoxin A in beer

被引：27

作者：

Goryacheva, I. Y. ^{[1
]}

Basova, E. Y. ^{[1
]}

Van Peteghem, C. ^{[2
]}

Eremin, S. A. ^{[3
]}

Pussemier, L. ^{[4
]}

Motte, J. -C. ^{[4
]}

De Saeger, S. ^{[2
]}

机构：

[1] Saratov NG Chernyshevskii State Univ, Fac Chem, Dept Common & Inorgan Chem, Saratov 410012, Russia

[2] Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium

[3] Moscow MV Lomonosov State Univ, Fac Chem, Dept Chem Enzymol, Moscow 119992, Russia

[4] Vet & Agrochem Res Ctr CODA CERVA, B-3080 Tervuren, Belgium

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 390卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

immunoassay; screening; mycotoxin; food control; visual detection; beer; ochratoxin A;

D O I：

10.1007/s00216-007-1713-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid easy-to-use immunoassay was optimised for the non-instrumental detection of ochratoxin A (OTA) in beer. The analytical method involves preconcentration on the immunoaffinity layer inside a column followed by direct competitive ELISA detection in the same layer. The visual cut-off value, i.e. the lowest OTA concentration resulting in no colour development, was 0.2 mu g L-1. Assay validation was performed using samples spiked with OTA. Thirty-seven naturally contaminated samples were screened with the gel-based method developed and no false-negative results were obtained. The method described offers a simple, rapid and cost-effective screening tool, thus contributing to better health protection of consumers.

引用

页码：723 / 727

页数：5

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