Determination of netilmicin sulfate by liquid chromatography with pulsed electrochemical detection

被引：31

作者：

Adams, E ^{[1
]}

Puelings, D ^{[1
]}

Rafiee, M ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farm Chem Anal Geneesmiddelen, B-3000 Leuven, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 812卷 / 1-2期

关键词：

pharmaceutical analysis; netilmicin sulfate; antibiotics; aminoglycosides; ethylgaramine; ethylnetilmicins; sisomicin;

D O I：

10.1016/S0021-9673(97)01250-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of netilmicin sulfate by Liquid chromatography using a column packed with poly(styrene-divinylbenzene) and pulsed electrochemical detection on a gold electrode is described. The mobile phase consisted of an aqueous solution containing 35 g l(-1) of sodium sulfate, 0.5 g l(-1) of sodium octanesulfonate, 10 ml l(-1) of tetrahydrofuran and 50 ml l(-1) of 0.2 M phosphate buffer (pH 3.0). The total analysis time was not more than 25 min. The effects of the different chromatographic parameters on the separation were also investigated. When a number of commercial samples of netilmicin sulfate was analyzed using this method, eight different components were separated, three of which were of unknown identities. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 157

页数：7

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