Spreadsheet-based program for the analysis of DNA methylation

被引：12

作者：

Anbazhagan, R ^{[1
]}

Herman, JG ^{[1
]}

Enika, K ^{[1
]}

Gabrielson, E ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21231 USA

来源：

BIOTECHNIQUES | 2001年 / 30卷 / 01期

关键词：

D O I：

10.2144/01301bc01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methylation of DNA in CpG dense regions of gene promoters (CpG islands) is important for transcriptional inactivation of selective genes in normal and neoplastic cells. Here, rte present a spreadsheet-based program adapted from Microsoft(R) Excel(R) that is useful for identifying CpG islands and for assisting in the laboratory analysis of DNA Methylation of these regions. Upon execution of the program, a customized workbook analyzes an entered DNA sequence for the total number and percentage cytosine and guanine nucleotides, the total number and percentage of CpG sires, and a CpG:GpC ratio. The program also displays the distribution of CpG sites in a visual format as well as in two different graphical formats. Finally the program assists in laboratory studies of DNA methylation that employ bisulfite modification of DNA by displaying methylation-dependent effects of bisulfite treatment on DNA sequences.

引用

页码：110 / +

页数：4

共 5 条

[1] Spreadsheet-based program for alignment of overlapping DNA sequences
Anbazhagan, R
Gabrielson, E
[J]. BIOTECHNIQUES, 1999, 26 (06) : 1180 - +
[2] Baylin SB, 1998, ADV CANCER RES, V72, P141
[3] CPG-RICH ISLANDS AND THE FUNCTION OF DNA METHYLATION
BIRD, AP
[J]. NATURE, 1986, 321 (6067) : 209 - 213
[4] CPG ISLANDS IN VERTEBRATE GENOMES
GARDINERGARDEN, M
FROMMER, M
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (02) : 261 - 282
[5] Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands
Herman, JG
Graff, JR
Myohanen, S
Nelkin, BD
Baylin, SB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (18) : 9821 - 9826

← 1 →