Characterization of an electrospray ionization ion trap linear time-of-flight mass spectrometer for phenylthiohydantoin amino acid analysis

被引：5

作者：

Gabryelski, W ^{[1
]}

Purves, RW ^{[1
]}

Li, L ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2C2, Canada

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 1998年 / 176卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

phenylthiohydantoin amino acid; Edman sequencing; electrospray ionization; ion-trap time-of-flight mass spectrometer;

D O I：

10.1016/S1387-3806(98)14032-0

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

An electrospray ionization ion trap/time-of-flight mass spectrometer was evaluated as a detector for identification of phenylthiohydantoin (PTH)-amino acids, the final products in the Edman sequencing process of peptides and proteins. Each of 22 PTH-amino acids studied can be characterized by well-defined mass spectral patterns. Fragmentation from source excitation followed by a long trapping period (250 ms) gave rise to unique and intense fragmentation products. This allowed for the differentiation of isomers of PTH-leucine/PTH-isoleucine and PTH-alpha-aminobutyric acid/PTH-alpha-aminoisobutyric acid. The interpretation of the fragmentation patterns is presented. The detection limits and relative detection sensitivity of 20 standard PTH-amino acids were determined. It is shown that all these compounds can be detected at the 100 fmol level. (C) 1998 Elsevier Science B.V.

引用

页码：213 / 225

页数：13

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