Pineapple (Ananas comosus Mess.) regeneration from leap explants

被引：3

作者：

Dolgov, SV ^{[1
]}

Shushkova, TV ^{[1
]}

Firsov, AP ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Bioorgan Chem, Pushchino 142292, Moscow Region, Russia

来源：

INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF TROPICAL AND SUBTROPICAL SPECIES - PART II | 1998年 / 461期

关键词：

pineapple; regeneration; in vitro culture;

D O I：

10.17660/ActaHortic.1998.461.51

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Pineapple (Ananas comosus Mess.) regeneration from in vitro leaf culture was achieved in 2 steps. The first step involved the formation of callus. Callus was observed on 23-30 % of explants. Its frequency depended upon plant age and which part of the leaf was cultured. At the second stage, callus formed plantlets at a frequency of 50-100%. Crumbly-globular callus with nodules produced more plantlets than firm-compact callus on MS media supplemented with 2iP, Kin, NAA and IBA. The best regeneration and callus formation were observed on leaf explants taken from in vitro plants 8-9 weeks old.

引用

页码：439 / 444

页数：6