Dynamics of competitive population abundance of Lactobacillus plantarum ivi gene mutants in faecal samples after passage through the gastrointestinal tract of mice

被引:30
作者
Bron, P. A.
Meijer, M.
Bongers, R. S.
De Vos, W. M.
Kleerebezem, M.
机构
[1] NIZO Food Res Hlth & Safety Dept, NL-6710 BA Ede, Netherlands
[2] Wageningen Ctr Food Sci, Microbial Funct & Safety Programme, Wageningen, Netherlands
关键词
competitive population abundance; gastrointestinal tract; ivi gene mutants; lactic acid bacteria; Lactobacillus plantarum;
D O I
10.1111/j.1365-2672.2007.03376.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aim: This study aims to evaluate the impact of mutation of previously identified in vivo- induced ( ivi) genes on the persistence and survival of Lactobacillus plantarum WCFS1 in the gastrointestinal ( GI) tract of mice. Methods and Results: Nine Lact. plantarum ivi gene replacement mutants were constructed, focussing on ivi genes that encode proteins with a predicted role in cell envelope functionality, stress response and regulation. The in vitro growth characteristics of the mutants appeared identical to those observed for the wild- type strain, which agrees with the recombination- based in vivo expression technology suggestion that these genes are not transcribed in the laboratory. Quantitative PCR experiments demonstrated differences in the relative population dynamics of the Lact. plantarum ivi mutants in faecal samples after passage through the GI tract of mice. Conclusions: The in situ competition experiments revealed a 100- to 1000- fold reduction of the relative abundance of three of the ivi gene mutants, harbouring deletions of genes predicted to encode a copper transporter, an orphan IIC cellobiose PTS and a cell wall anchored extracellular protein. Significance and Impact of the Study: These experiments clearly establish that the proteins encoded by these three genes play a key role in Lact. plantarum performance during passage of the GI tract.
引用
收藏
页码:1424 / 1434
页数:11
相关论文
共 34 条
[1]   In vivo expression technology [J].
Angelichio, MJ ;
Carnilli, A .
INFECTION AND IMMUNITY, 2002, 70 (12) :6518-6523
[2]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[3]   Genome-wide detection and analysis of cell wall-bound proteins with LPxTG-Like sorting motifs [J].
Boekhorst, J ;
de Been, MWHJ ;
Kleerebezem, M ;
Siezen, RJ .
JOURNAL OF BACTERIOLOGY, 2005, 187 (14) :4928-4934
[4]   Identification of Lactobacillus plantarum genes that are induced in the gastrointestinal tract of mice [J].
Bron, PA ;
Grangette, C ;
Mercenier, A ;
de Vos, WM ;
Kleerebezem, M .
JOURNAL OF BACTERIOLOGY, 2004, 186 (17) :5721-5729
[5]   Genetic characterization of the bile salt response in Lactobacillus plantarum and analysis of responsive promoters in vitro and in situ in the gastrointestinal tract [J].
Bron, PA ;
Marco, M ;
Hoffer, SM ;
Van Mullekom, E ;
de Vos, WM ;
Kleerebezem, M .
JOURNAL OF BACTERIOLOGY, 2004, 186 (23) :7829-7835
[6]   ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI [J].
CASADABAN, MJ ;
COHEN, SN .
JOURNAL OF MOLECULAR BIOLOGY, 1980, 138 (02) :179-207
[7]   LACTOBACILLUS-PLANTARUM LDHL GENE - OVEREXPRESSION AND DELETION [J].
FERAIN, T ;
GARMYN, D ;
BERNARD, N ;
HOLS, P ;
DELCOUR, J .
JOURNAL OF BACTERIOLOGY, 1994, 176 (03) :596-601
[8]   Mutants in the CtpA copper transporting P-type ATPase reduce virulence of Listeria monocytogenes [J].
Francis, MS ;
Thomas, CJ .
MICROBIAL PATHOGENESIS, 1997, 22 (02) :67-78
[9]   The Listeria monocytogenes gene ctpA encodes a putative P-type ATPase involved in copper transport [J].
Francis, MS ;
Thomas, CJ .
MOLECULAR & GENERAL GENETICS, 1997, 253 (04) :484-491
[10]   The use of listeriolysin to identify in vivo induced genes in the Gram-positive intracellular pathogen Listeria monocytogenes [J].
Gahan, CGM ;
Hill, C .
MOLECULAR MICROBIOLOGY, 2000, 36 (02) :498-507