Growth kinetics, diffraction properties and effect of agarose on the stability of a novel crystal form of Thermus thermophilus aspartyl-tRNA synthetase-1

被引：37

作者：

Zhu, DW

Lorber, B

Sauter, C

Ng, JD

Bénas, P

Le Grimellec, C

Giegé, R

机构：

[1] CNRS, Inst Biol Mol & Cellulaire, UPR 9002, Dept Mecanismes & Macromol Synth Prot, F-67084 Strasbourg, France

[2] INSERM, U414, Ctr Biochim Struct, F-34090 Montpellier, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷

关键词：

D O I：

10.1107/S0907444901001743

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Growth kinetics and diffraction properties of monoclinic crystals of eubacterial Thermus thermophilus aspartyl-tRNA synthetase-1 (AspRS-1) prepared in the presence of polyethylene glycol and agarose are studied. Their solubility and two-dimensional phase diagram are compared with those of orthorhombic crystals which grow in the presence of sodium formate or ammonium sulfate. The growth mechanism of the novel crystals was monitored by atomic force microscopy. The gel stabilizes the crystal lattice under the cryogenic conditions used for structure determination at high resolution.

引用

页码：552 / 558

页数：7

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