The P2 protein of rice dwarf phytoreovirus is required for adsorption of the virus to cells of the insect vector

被引:41
作者
Omura, T [1 ]
Yan, J [1 ]
Zhong, BX [1 ]
Wada, M [1 ]
Zhu, YF [1 ]
Tomaru, M [1 ]
Maruyama, W [1 ]
Kikuchi, A [1 ]
Watanabe, Y [1 ]
Kimura, I [1 ]
Hibino, H [1 ]
机构
[1] Natl Agr Res Ctr, Tsukuba, Ibaraki 305, Japan
关键词
D O I
10.1128/JVI.72.11.9370-9373.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Intact particles of rice dwarf phytoreovirus adsorbed to and entered monolayer-cultured cells of the insect vector Nephotettix cincticeps and multiplied within the cells. Particles that lacked the PZ protein neither attached to nor infected such cells. Furthermore, P2-free particles obtained from a transmission-competent isolate of the virus were unable to infect insect vectors that had been allowed to feed on these virus particles through a membrane. However, when such virus particles were injected into insects via a glass capillary tube they successfully infected the insects, which became able to transmit the virus, These results support the hypothesis that, while P2-free particles can neither interact with nor infect cells in the intestinal tract of the insect vector, they do retain the ability to infect such cells when physically introduced into the hemolymph by injection.
引用
收藏
页码:9370 / 9373
页数:4
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