Simultaneous Analysis of Glycosylated and Sialylated Prostate-Specific Antigen Revealing Differential Distribution of Glycosylated Prostate-Specific Antigen Isoforms in Prostate Cancer Tissues

被引：72

作者：

Li, Yan ^{[1
]}

Tian, Yuan ^{[1
]}

Rezai, Taha ^{[2
]}

Prakash, Amol ^{[2
]}

Lopez, Mary F. ^{[2
]}

Chan, Daniel W. ^{[1
]}

Zhang, Hui ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Pathol, Baltimore, MD 21287 USA

[2] Thermo Fisher BRIMS, Cambridge, MA 02139 USA

来源：

ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 01期

基金：

美国国家卫生研究院;

关键词：

PROTEIN-GLYCAN INTERACTIONS; TANDEM MASS-SPECTROMETRY; SEMINAL PLASMA; ABSOLUTE QUANTIFICATION; ALTERED GLYCOSYLATION; LIQUID-CHROMATOGRAPHY; PSA; DISCOVERY; BIOMARKER; SERUM;

D O I：

10.1021/ac102319g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aberrant protein glycosylation has been shown to be associated with disease progression and can be potentially useful as a biomarker if disease-specific glycosylation can be identified. However, high-throughput quantitative analysis of protein glycosylation derived from clinical specimens presents technical challenges due to the typically high complexity of biological samples. In this study, a mass spectrometry-based analytical method was developed to measure different glycosylated forms of glycoproteins from complex biological samples by coupling glycopeptide extraction strategy for specific glycosylation with selected reaction monitoring (SRM). Using this method, we monitored glycosylated and sialylated prostate-specific antigen (PSA) in prostate cancer and noncancer tissues. Results of this study demonstrated that the relative abundance of glycosylated PSA isoforms were not correlated with total PSA protein levels measured in the same prostate cancer tissue samples by clinical immunoassay. Furthermore, the sialylated PSA was differentially distributed in cancer and noncancer tissues. These data suggest that differently glycosylated isoforms of glycoproteins can be quantitatively analyzed and may provide unique information for clinically relevant studies.

引用

页码：240 / 245

页数：6

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