Coat protein-mediated protection to cucumber mosaic virus infections in cultivated tomato

被引：24

作者：

Gielen, J ^{[1
]}

Ultzen, T ^{[1
]}

Bontems, S ^{[1
]}

Loots, W ^{[1
]}

vanSchepen, A ^{[1
]}

Westerbroek, A ^{[1
]}

deHaan, P ^{[1
]}

vanGrinsven, M ^{[1
]}

机构：

[1] S&G SEMENCES SA,CTR RECH,F-84260 SARRIANS,FRANCE

来源：

EUPHYTICA | 1996年 / 88卷 / 02期

关键词：

coat protein; cucumber mosaic virus; Lycopersicon; tomato; transformation; virus resistance;

D O I：

10.1007/BF00032445

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Cucumber mosaic virus (CMV) infections rank among the most devastating diseases in the commercial culture of tomato (Lycopersicon esculentum Mill.), for which suitable sources of natural resistance are not available. The concept of pathogen-derived resistance, however, offers an alternate approach to combat plant viral diseases by transformation of crops with nucleotide sequences derived from the viral genome. This report demonstrates the successful application of such a pathogen-derived resistance gene comprising the CMV coat protein (CP) gene, to generate protection to CMV infections in cultivated tomato. Transformation of an inbred tomato line with the CMV CP gene isolated from a subgroup I strain, engendered high levels of protection to various CMV strains, including a virulent strain causing lethal necrosis and a typical subgroup II strain. Moreover, when challenged by natural infection through aphid vectors in open field, levels of protection were largely maintained in hemizygous hybrids. In all, these results demonstrate that synthetic resistance genes based on the CMV CP gene make excellent sources of broad spectrum resistance to CMV infections for introgression into tomato breeding programs.

引用

页码：139 / 149

页数：11

共 37 条

[1] DELAY OF DISEASE DEVELOPMENT IN TRANSGENIC PLANTS THAT EXPRESS THE TOBACCO MOSAIC-VIRUS COAT PROTEIN GENE [J].