A widely distributed ITS polymorphism within a biological species of the ectomycorrhizal fungus Hebeloma velutipes

被引:51
作者
Aanen, DK
Kuyper, TW
Hoekstra, RF
机构
[1] Univ Wageningen & Res Ctr, Genet Lab, NL-6703 HA Wageningen, Netherlands
[2] Univ Wageningen & Res Ctr, Dept Environm Sci, Sect Soil Qual, NL-6700 EC Wageningen, Netherlands
来源
MYCOLOGICAL RESEARCH | 2001年 / 105卷
关键词
D O I
10.1017/S0953756201003628
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The ectomycorrhizal fungus Hebeloma velutipes consists of two biological species (BSP 16 and 17). Within BSP 17 a dikaryon was found with two divergent types of the ribosomal Internal Transcribed Spacer (ITS1 and 2). The two ITS types segregated in monokaryotic progeny of that dikaryon, showing that these different ITS types represent different alleles at homologous rDNA loci in the two nuclei. RFLP analysis of a number of strains of BSP 17 showed that the polymorphism is widespread in Europe. There was no deficiency of the heterokaryotic type, demonstrating that ITS divergence in this species is not correlated with reduced intercompatibility. A strain from North America, not assigned to a biological species, showed the same polymorphism. Cladistic analysis of the two ITS sequences showed that they were not sister groups. One of the ITS types formed a monophyletic group together with the ITS type of BSP 16, the other type formed a clade with the ITS type of H. incarnatulum (BSP 18). BSP 16 and 17 showed partial intercompatibility. However, several lines of evidence suggest that the polymorphism of BSP 17 is not the result of frequent and continuing hybridisation with BSP 16. Instead, we give arguments for the hypothesis that the polymorphism evolved in allopatry and that the two types have come together relatively recently. The results of the polymorphism indicate a potential problem for molecular identification of fungal species based on ITS fingerprinting. The results also show that no generalisations are possible about the relation of speciation (the formation of BSP) and nuclear ITS divergence.
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页码:284 / 290
页数:7
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共 28 条
  • [1] Intercompatibility tests in the Hebeloma crustuliniforme complex in northwestern Europe
    Aanen, DK
    Kuyper, TW
    [J]. MYCOLOGIA, 1999, 91 (05) : 783 - 795
  • [2] Phylogenetic relationships in the genus Hebeloma based on ITS1 and 2 sequences, with special emphasis on the Hebeloma crustuliniforme complex
    Aanen, DK
    Kuyper, TW
    Boekhout, T
    Hoekstra, RF
    [J]. MYCOLOGIA, 2000, 92 (02) : 269 - 281
  • [3] Aanen DK, 2000, EVOLUTION, V54, P1192, DOI 10.1111/j.0014-3820.2000.tb00554.x
  • [4] MOLECULAR EVIDENCE FOR GENETIC EXCHANGES AMONG RIBOSOMAL GENES ON NON-HOMOLOGOUS CHROMOSOMES IN MAN AND APES
    ARNHEIM, N
    KRYSTAL, M
    SCHMICKEL, R
    WILSON, G
    RYDER, O
    ZIMMER, E
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12): : 7323 - 7327
  • [5] THE ITS REGION OF NUCLEAR RIBOSOMAL DNA - A VALUABLE SOURCE OF EVIDENCE ON ANGIOSPERM PHYLOGENY
    BALDWIN, BG
    SANDERSON, MJ
    PORTER, JM
    WOJCIECHOWSKI, MF
    CAMPBELL, CS
    DONOGHUE, MJ
    [J]. ANNALS OF THE MISSOURI BOTANICAL GARDEN, 1995, 82 (02) : 247 - 277
  • [6] BREMER K, 1988, EVOLUTION, V42, P795, DOI [10.2307/2408870, 10.1111/j.1558-5646.1988.tb02497.x]
  • [7] BRUCHET G, 1970, Bulletin Mensuel de la Societe Linneenne de Lyon, V39, P3
  • [8] Zea systematics: Ribosomal ITS evidence
    Buckler, ES
    Holtsford, TP
    [J]. MOLECULAR BIOLOGY AND EVOLUTION, 1996, 13 (04) : 612 - 622
  • [9] PHYLOGENETIC-RELATIONSHIPS OF DIPSACALES BASED ON RBCL SEQUENCES
    DONOGHUE, MJ
    OLMSTEAD, RG
    SMITH, JF
    PALMER, JD
    [J]. ANNALS OF THE MISSOURI BOTANICAL GARDEN, 1992, 79 (02) : 333 - 345
  • [10] CONCERTED EVOLUTION OF REPETITIVE DNA-SEQUENCES IN EUKARYOTES
    ELDER, JF
    TURNER, BJ
    [J]. QUARTERLY REVIEW OF BIOLOGY, 1995, 70 (03) : 297 - 320