Determination of the primary structure of two lipid transfer proteins from apricot (Prunus armeniaca)

It has been recently demonstrated that the major allergen of apricot is a protein of molecular mass (M-r) 9000 belonging to the family of Lipid Transfer Protein. The aim of this study was the determination of the primary structure of apricot LTP by micro-sequencing and mass spectrometric analyses. Apricot LTP is a 91 amino acids protein like peach and almond LTPs with a sequence identity of 91% and 94%, respectively. Like for the peach LTP, out of the 25 amino acids forming the inner surface of the tunnel-like hydrophobic cavity in maize ns-LTP, 16 are identical and 7 similar in the apricot LTP, supporting the hypothesis of a similar function. (C) 2001 Elsevier Science B.V. All rights reserved.