Glucosylation of isatin-3-oxime followed by 2D in situ nmr in plant cells at highest magnetic field without labelling

被引：4

作者：

Hinse, C

Unger, M

Provenzani, A

Stöckigt, J

机构：

[1] Univ Mainz, Inst Pharm, Dept Pharmaceut Biol, D-55099 Mainz, Germany

[2] Univ Florence, Ctr Risonanze Magnet, I-50019 Florence, Italy

来源：

NATURAL PRODUCT LETTERS | 2001年 / 15卷 / 02期

关键词：

isatin-3-oxime; glucosylation; 2D in situ NMR; Rauvolfia serpentina; cell suspension cultures;

D O I：

10.1080/10575630108041268

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glucosylation of isatin-3-oxime (1) was monitored by in situ 2D H-1-C-13 inverse correlated gradient assisted NMR spectroscopy in plant cell suspension cultures of Rauvolfia serpentina without labelling. The applied high magnetic field of 800 MHz allowed measurements within 20 min at concentrations of 1 of 5.76 mM. Complete glucosylation of 1 occurs inside the cells within 72 hours. During this time isatin-3-oxime-glucoside (2) accumulates without further metabolism.

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页码：119 / 124

页数：6

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