Inactivation of Yersinia enterocolitica by pulsed electric fields

被引：54

作者：

Alvarez, I ^{[1
]}

Raso, J ^{[1
]}

Sala, FJ ^{[1
]}

Condón, S ^{[1
]}

机构：

[1] Univ Zaragoza, Fac Vet, E-50013 Zaragoza, Spain

来源：

FOOD MICROBIOLOGY | 2003年 / 20卷 / 06期

关键词：

Yersinia; pulsed electric fields; Weibull;

D O I：

10.1016/S0740-0020(03)00033-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The influence of growth conditions, treatment medium characteristics and PEF process parameters on the lethal effect on Yersinia enterocolitica of pulsed electric fields (PEF) treatments in batch has been investigated. Growth phase, temperature of growth, pH, conductivity of the treatment medium, pulse width and frequency of pulses did not influence the sensitivity of Y enterocolitica to PEF. However, an A(w) decrease from > 0.99 to 0.93 of the treatment medium increased the PEF resistance of Y enterocolitica with 3.5 log(10) cycles after a treatment of 22 kV/cm, 800 mus and 880 kJ/kg. Inactivation of Y enterocolitica increased with the field strength, treatment time and total specific energy up to a maximum of 6 log(10) cycles after 28 kV/cm, 2000 mus and 3559 kJ/kg. A nonlinear relationship was found among the survival fraction and the treatment time or the specific energy that was accurately described by a mathematical model based on the Weibull distribution. The inactivation of Y. enterocolitica by PEF was characterized by maximum field strength thresholds. Above these thresholds, specific energy necessary to obtain a given level of inactivation scarcely decreased by increasing the electric field strength, and inactivation of Y. enterocolitica only depended on the specific energy applied. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：691 / 700

页数：10

共 43 条

[1]

ALVAEZ I, 2002, UNPUB LETT APPL MICR

[2] Influence of different factors on the inactivation of Salmonella senftenberg by pulsed electric fields [J].