Purification and identification of a novel heteropolysaccharide RBPS2a with anti-complementary activity from defatted rice bran

A novel heteropolysaccharide RBPS2a with anti-complementary activity was obtained from defatted rice bran by hot water extraction, ethanol precipitation, and purified by gel chromatography after anion-exchange chromatography. This fraction exhibited more potent anti-complementary activity than other polysaccharide fractions. RBPS2a was eluted as a single symmetrical narrow peak on high-performance gel-permeation chromatography (HPGPC) and the average molecular weight was 90,000 Da. We found RBPS2a contained 86.7% polysaccharide and 8.7% protein. The amino acid pattern showed that RBPS2a contained large amount of glutamic acid, arginine, aspartic acid, lysine, and alanine. The molar content of the above five amino acids constituted 59.31% of the total amino acids. Gas chromatography of absolute acid hydrolysate of RBPS2a suggested that it was composed of arabinose, xylose, glucose and galactose with a molar ratio of 4:2:1:4. The Fourier-transform infrared spectra (FT-IR) and H-1, C-13 NMR spectroscopy analysis revealed that RBPS2a had a backbone consisting of beta-(1 -> 3)-linked D-galacopyranosyl residues substituted at O-2 with glycosyl residues composed of alpha-D-xylose-(1 -> 4)-alpha-D-arabinose-(1 -> and alpha-D-glucose-(1 -> 4)-alpha-D-arabinose-(1 -> linked residues. Furthermore, some of the fractions extracted and purified from defatted rice bran exhibited strong anti-complementary activity. Among these fractions, the purified polysaccharide RBPS2a had the highest activity. (c) 2008 Elsevier Ltd. All rights reserved.