Impaired E-cadherin expression and glutamine synthetase overexpression in solid pseudopapillary neoplasm of the pancreas

被引：42

作者：

Audard, Virginie ^{[3
,4
]}

Cavard, Catherine ^{[3
,4
]}

Richa, Hubert ^{[5
]}

Infante, Muriel ^{[6
]}

Couvelard, Anne ^{[7
]}

Sauvanet, Alain ^{[8
]}

Terris, Benoit ^{[1
,4
,9
]}

Paye, Francois ^{[2
]}

Flejou, Jean-Francois ^{[7
]}

机构：

[1] Hop Cochin, Serv Anat Pathol, AP HP, Dept Pathol, F-75679 Paris 14, France

[2] Hop St Antoine, AP HP, Dept Digest Surg, F-75571 Paris, France

[3] Univ Paris 05, CNRS, Inst Cochin, Paris, France

[4] INSERM, Paris, France

[5] Hop Conception, AP HM, Dept Surg & Liver Transplantat, Marseille, France

[6] Hop St Antoine, Dept Pathol, F-75571 Paris, France

[7] Hop Beaujon, AP HP, Dept Pathol, Clichy, France

[8] Hop Beaujon, AP HP, Dept Digest Surg, Clichy, France

[9] Univ Paris 05, Fac Med Rene Descartes, Paris, France

来源：

PANCREAS | 2008年 / 36卷 / 01期

关键词：

solid pseudopapillary neoplasm of the pancreas; beta-catenin; E-cadherin; glutamine synthetase;

D O I：

10.1097/mpa.0b013e318137a9da

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Objectives: To analyze in solid pseudopapillary neoplasm of the pancreas (SPNP) the consequences of the deregulated Wnt pathway by studying the expression of Wnt target glutamine synthetase (GLUL), cyclin D1, and E-cadherin, which is one of the beta-catenin binding partner in cell adhesion. Methods: The expression of cyclin D1 and GLUL was studied at the protein and/or messenger RNA levels, and the immunolocalization for E-cadherin was analyzed in 28 SPNPs screened for beta-catenin mutations. Expression of cyclin D1, GLUL, and beta-catenin was also assessed in pancreatic endocrine tumors as controls. Results: Cytosolic and/or nuclear accumulation of beta-catenin was observed in all tumors; an activating beta-catenin mutation was identified in 21 (91%) of 23 tumors analyzed. E-cadherin expression is lost from the membrane and is observed in intracytosolic "dotlike" structures. Whereas cyclin D1 expression is observed widely in SPNP and endocrine tumors, GLUL expression is restricted to SPNP (100%) and rare endocrine tumors (10%) displaying Wnt activation. Conclusions: The activation of the Wnt/beta-catenin pathway in SPNP has 2 main consequences. First, E-cadherin expression moved from membranous to intracytoplasmic localization. Second, GLUL expression is highly correlated with Wnt/beta-catenin activation, demonstrating its faithfulness as a Wnt target gene.

引用

页码：80 / 83

页数：4

共 23 条

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