Mass Analysis of Biological Macromolecules at Atmospheric Pressure Using Nonresonant Femtosecond Laser Vaporization and Electrospray Ionization

被引：28

作者：

Judge, Elizabeth J. ^{[1
]}

Brady, John J. ^{[1
]}

Levis, Robert J. ^{[1
]}

机构：

[1] Temple Univ, Dept Chem, Philadelphia, PA 19122 USA

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 24期

基金：

美国国家科学基金会;

关键词：

AMBIENT CONDITIONS; SPECTROMETRY; DESORPTION; EGG; TIME; DESORPTION/IONIZATION; PHOSPHOLIPIDS; BIOMOLECULES; INTACT;

D O I：

10.1021/ac102409k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A nonresonant femtosecond laser pulse, with an intensity of 10(13) Wcm(-2), vaporizes proteins and biomolecules intact, regardless of molecular structure, size or electronic structure for subsequent electrospray ionization and transfer into a mass spectrometer. Rapid, direct analysis from dried sample, aqueous solution and cellular material is demonstrated at atmospheric pressure using laser electrospray mass spectrometry (LEMS). Measurements are presented for lysozyme (14.3 kDa), hemoglobin from human blood, ovalbumin (45 kDa) from hen egg white and phospholipids from hen egg yolk. Mass analysis of biological material is performed without dilution, extraction or sample preparation, other than placing the biological material onto the sample plate.

引用

页码：10203 / 10207

页数：5

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