DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR

被引：65

作者：

Cogswell, FB ^{[1
]}

Bantar, CE ^{[1
]}

Hughes, TG ^{[1
]}

Gu, Y ^{[1
]}

Philipp, MT ^{[1
]}

机构：

[1] TULANE UNIV,TULANE PRIMATE RES CTR,MED CTR,DEPT PARASITOL,COVINGTON,LA 70433

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 04期

关键词：

D O I：

10.1128/JCM.34.4.980-982.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

It is demonstrated that a diagnostic PCR for Borrelia burgdorferi can be inhibited in the presence of more than 500 ng of host (monkey skin) DNA. The Inhibitor is the host DNA itself. An acceptable value for analytical sensitivitg can be obtained by diluting the skin-B. burgdorferi proteinase K lysate to a level below the inhibitory concentration of the host DNA. Dilution of the lysate may obviate the need for further DNA purification.

引用

页码：980 / 982

页数：3

共 14 条

[1] CHIVARA R, 1984, ANAL BIOCHEM, V136, P446
[2] BLOOD-STREAM INVASION IN EARLY LYME-DISEASE - RESULTS FROM A PROSPECTIVE, CONTROLLED, BLINDED STUDY USING THE POLYMERASE CHAIN-REACTION
GOODMAN, JL
BRADLEY, JF
ROSS, AE
GOELLNER, P
LAGUS, A
VITALE, B
BERGER, BW
LUGER, S
JOHNSON, RC
[J]. AMERICAN JOURNAL OF MEDICINE, 1995, 99 (01) : 6 - 12
[3] KITTEN T, 1992, GENETICS, V132, P311
[4] ISOLATION OF BORRELIA-BURGDORFERI FROM BIOPSY SPECIMENS TAKEN FROM HEALTHY-LOOKING SKIN OF PATIENTS WITH LYME BORRELIOSIS
KUIPER, H
VANDAM, AP
SPANJAARD, L
DEJONGH, BM
WIDJOJOKUSUMO, A
RAMSELAAR, TCP
CAIRO, I
VOS, K
DANKERT, J
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (03) : 715 - 720
[5] DETECTION OF BORRELIA-BURGDORFERI SENSU-LATO IN LESIONAL SKIN OF PATIENTS WITH ERYTHEMA MIGRANS AND ACRODERMATITIS CHRONICA ATROPHICANS BY OSPA-SPECIFIC PCR
MOTER, SE
HOFMANN, H
WALLICH, R
SIMON, MM
KRAMER, MD
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (12) : 2980 - 2988
[6] TARGET IMBALANCE - DISPARITY OF BORRELIA-BURGDORFERI GENETIC MATERIAL IN SYNOVIAL-FLUID FROM LYME ARTHRITIS PATIENTS
PERSING, DH
RUTLEDGE, BJ
RYS, PN
PODZORSKI, DS
MITCHELL, PD
REED, KD
LIU, B
FIKRIG, E
MALAWISTA, SE
[J]. JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (03) : 668 - 672
[7] EARLY AND EARLY DISSEMINATED PHASES OF LYME-DISEASE IN THE RHESUS-MONKEY - A MODEL FOR INFECTION IN HUMANS
PHILIPP, MT
AYDINTUG, MK
BOHM, RP
COGSWELL, FB
DENNIS, VA
LANNERS, HN
LOWRIE, RC
ROBERTS, ED
CONWAY, MD
KARACORLU, M
PEYMAN, GA
GUBLER, DJ
JOHNSON, BJB
PIESMAN, J
GU, Y
[J]. INFECTION AND IMMUNITY, 1993, 61 (07) : 3047 - 3059
[8] SUCCESSFUL AMPLIFICATION OF DNA SPECIFIC FOR FINNISH BORRELIA-BURGDORFERI ISOLATES IN ERYTHEMA CHRONICUM MIGRANS BUT NOT IN CIRCUMSCRIBED SCLERODERMA LESIONS
RANKI, A
AAVIK, E
PETERSON, P
SCHAUMAN, K
NURMILAAKSO, P
[J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 102 (03) : 339 - 345
[9] ROBERTS ED, 1995, LAB INVEST, V72, P146
[10] Salinas-Melendez J. A., 1995, Revista Latinoamericana de Microbiologia, V37, P7

← 1 2 →