Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts

被引：84

作者：

Guadagno, TM ^{[1
]}

Ferrell, JE ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Mol Pharmacol, Stanford, CA 94305 USA

来源：

SCIENCE | 1998年 / 282卷 / 5392期

关键词：

D O I：

10.1126/science.282.5392.1312

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The p42 mitogen-activated protein kinase (MAPK) is required for progression through meiotic M phase in Xenopus oocytes. This report examines whether it also plays a role in normal mitotic progression. MAPK was transiently activated during mitosis in cycling Xenopus egg extracts after activation of the cyclin-dependent kinase Cdc2-cyclin B. Interference with MAPK activation by immunodepletion of its activator MEK, or by addition of the MEK inhibitor PD98059, caused precocious termination of mitosis and interfered with production of normal mitotic microtubules. Sustained activation of MAPK arrested extracts in mitosis in the absence of active Cdc2-cyclin B. These findings identify a role for MEK and MAPK in maintaining the mitotic state.

引用

页码：1312 / 1315

页数：4

共 29 条

[1] MAPK inactivation is required for the G(2) to M-phase transition of the first mitotic cell cycle [J].

Abrieu, A ;

Fisher, D ;

Simon, MN ;

Doree, M ;

Picard, A .

EMBO JOURNAL, 1997, 16 (21) :6407-6413

[2] Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis [J].

Acharya, U ;

Mallabiabarrena, A ;

Acharya, JK ;

Malhotra, V .

CELL, 1998, 92 (02) :183-192

[3] PD-098059 IS A SPECIFIC INHIBITOR OF THE ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASE KINASE IN-VITRO AND IN-VIVO [J].

ALESSI, DR ;

CUENDA, A ;

COHEN, P ;

DUDLEY, DT ;

SALTIEL, AR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (46) :27489-27494

[4] Activation of the p42 mitogen-activated protein kinase pathway inhibits Cdc2 activation and entry into M-phase in cycling Xenopus egg extracts [J].

Bitangcol, JC ;

Chau, ASS ;

Stadnick, E ;

Lohka, MJ ;

Dicken, B ;

Shibuya, EK .

MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (02) :451-467

[5]

Chiri S, 1998, J CELL SCI, V111, P2519

[6] PD98059 prevents establishment of the spindle assembly checkpoint and inhibits the G2-M transition in meiotic but not mitotic cell cycles in Xenopus [J].

Cross, DAE ;

Smythe, C .

EXPERIMENTAL CELL RESEARCH, 1998, 241 (01) :12-22

[7] SUPPRESSION OF DNA-REPLICATION VIA MOS FUNCTION DURING MEIOTIC DIVISIONS IN XENOPUS-OOCYTES [J].

FURUNO, N ;

NISHIZAWA, M ;

OKAZAKI, K ;

TANAKA, H ;

IWASHITA, J ;

NAKAJO, N ;

OGAWA, Y ;

SAGATA, N .

EMBO JOURNAL, 1994, 13 (10) :2399-2410

[8] INVITRO EFFECTS ON MICROTUBULE DYNAMICS OF PURIFIED XENOPUS M-PHASE-ACTIVATED MAP KINASE [J].

GOTOH, Y ;

NISHIDA, E ;

MATSUDA, S ;

SHIINA, N ;

KOSAKO, H ;

SHIOKAWA, K ;

AKIYAMA, T ;

OHTA, K ;

SAKAI, H .

NATURE, 1991, 349 (6306) :251-254

[9]

GUADAGNO TM, UNPUB

[10] EVIDENCE THAT INACTIVE P42 MITOGEN-ACTIVATED PROTEIN-KINASE AND INACTIVE RSK EXIST AS A HETERODIMER IN-VIVO [J].

HSIAO, KM ;

CHOU, SY ;

SHIH, SJ ;

FERRELL, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (12) :5480-5484

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