PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis

被引：455

作者：

Graves, LM ^{[1
]}

Swaminathan, B ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Foodbourne & Diarrheal Dis Branch, Div Bacterial & Mycot Dis, Natl Ctr Infect Dis, Atlanta, GA 30333 USA

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2001年 / 65卷 / 1-2期

关键词：

PulseNet; Listeria monocytogenes; pulsed-field gel electrophoresis; subtyping; epidemiology;

D O I：

10.1016/S0168-1605(00)00501-8

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

PulseNet is a national network of pubic health and food regulatory laboratories established in the US to detect clusters of foodborne disease and respond quickly to foodborne outbreak investigations. PulseNet laboratories: currently subtype Escherichia coli O157:H7, non-typhoidal Salmonella. and Shigella isolates by a highly standardized I-day pulsed-field gel electrophoresis (PFGE). and exchange normalized DNA "fingerprint" patterns via the Internet. We describe a standardized molecular subtyping protocol for subtyping Listeria monocytogenes that was recently added to PulseNet. The subtyping can be completed within 30 h from the time a pure culture of the bacteria is obtained. (C) 2001 Elsevier science B.V. All rights reserved.

引用

页码：55 / 62

页数：8

共 21 条

[1]

[Anonymous], 1999, MMWR, V47, P1117

[2] WHO International Multicenter Listeria monocytogenes Subtyping Study - Rationale and set-up of the study [J].