Utility of the Citrobacter rodentium infection model in laboratory mice

被引:113
作者
Borenshtein, Diana [1 ]
McBee, Megan E. [1 ]
Schauer, David B. [1 ]
机构
[1] MIT, Dept Biol Engn, Div Comparat Med, Cambridge, MA 02139 USA
关键词
acute diarrheal illness; Citrobacter freundii biotype 4280; colitis; Enterobacteriaceae infections; transmissible murine colonic hyperpiasia;
D O I
10.1097/MOG.0b013e3282f2b0fb
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Purpose of review There have been considerable advances in our understanding of the molecular pathogenesis of enteropathogenic Escherichia coli and enterohemorrhagic E coli infection. Given the difficulty of infecting laboratory mice with these diarrhea-causing pathogens, a growing number of studies have found the murine bacterial pathogen Citrobacter rodentium to provide a robust, relevant in-vivo model system. Recent findings All inbred strains and outbred stocks of laboratory mice studied to date have been found to be susceptible to C. rodentium infection. The natural course of disease ranges from subclinical epithelial hyperplasia in the colon, to clinical diarrhea and colitis, to fatal infection, depending on the age, genetic background, and health status of the host. Infection is self-limiting, leading to disease resolution and protective immunity. Here we review recent discoveries related to bacterial virulence determinants, epithelial hyperplasia, innate and adaptive immune responses, and mechanisms of diarrhea. Summary Infection of laboratory mice with C. rodentium provides a useful in-vivo model for studying the pathogenesis of infectious gastroenteritis and acute diarrheal illness, and for preclinical evaluation of candidate preventive and therapeutic agents.
引用
收藏
页码:32 / 37
页数:6
相关论文
共 46 条
[1]  
BARTHOLD SW, 1977, LAB ANIM SCI, V27, P938
[2]   Development of fatal colitis in FVB mice infected with Citrobacter rodentium [J].
Borenshtein, Diana ;
Nambiar, Prashant R. ;
Groff, Elizabeth B. ;
Fox, James G. ;
Schauer, David B. .
INFECTION AND IMMUNITY, 2007, 75 (07) :3271-3281
[3]   CD4+-T-cell effector functions and costimulatory requirements essential for surviving mucosal infection with Citrobacter rodentium [J].
Bry, L ;
Brigl, M ;
Brenner, MB .
INFECTION AND IMMUNITY, 2006, 74 (01) :673-681
[4]   Critical role of T cell-dependent serum antibody, but not the gut-associated lymphoid tissue, for surviving acute mucosal infection with Citrobacter rodentium, an attaching and effacing pathogen [J].
Bry, L ;
Brenner, MB .
JOURNAL OF IMMUNOLOGY, 2004, 172 (01) :433-441
[5]   Helminth-primed dendritic cells alter the host response to enteric bacterial infection [J].
Chen, CC ;
Louie, S ;
McCormick, BA ;
Walker, WA ;
Shi, HN .
JOURNAL OF IMMUNOLOGY, 2006, 176 (01) :472-483
[6]   Preinoculation with the probiotic Lactobacillus acidophilus early in life effectively inhibits murine Citrobacter rodentium colitis [J].
Chen, CC ;
Louie, S ;
Shi, HN ;
Walker, WA .
PEDIATRIC RESEARCH, 2005, 58 (06) :1185-1191
[7]   Concurrent infection with an intestinal helminth parasite impairs host resistance to enteric Citrobacter rodentium and enhances Citrobacter-induced colitis in mice [J].
Chen, CC ;
Louie, S ;
McCormick, B ;
Walker, WA ;
Shi, HN .
INFECTION AND IMMUNITY, 2005, 73 (09) :5468-5481
[8]   Bacillus subtilis spores reduce susceptibility to Citrobacter rodentium-mediated enteropathy in a mouse model [J].
D'Arienzo, Rossana ;
Maurano, Francesco ;
Mazzarella, Giuseppe ;
Luongo, Diomira ;
Stefanile, Rosita ;
Ricca, Ezio ;
Rossi, Mauro .
RESEARCH IN MICROBIOLOGY, 2006, 157 (09) :891-897
[9]   Animal models of inflammatory bowel disease - Lessons from enteric infections [J].
Eckmann, Lars .
INFLAMMATORY BOWEL DISEASE: GENETICS, BARRIER FUNCTION, IMMUNOLOGIC MECHANISMS, AND MICROBIAL PATHWAYS, 2006, 1072 :28-38
[10]   Attaching and effacing pathogen-induced tight junction disruption in vivo [J].
Guttman, JA ;
Li, YL ;
Wickham, ME ;
Deng, WY ;
Vogl, AW ;
Finlay, BB .
CELLULAR MICROBIOLOGY, 2006, 8 (04) :634-645