Expression of transforming growth factor β isoforms and their roles in tendon healing

被引：56

作者：

Chan, Kai-Ming ^{[1
]}

Fu, Sai-Chuen ^{[1
]}

Wong, Yim-Ping ^{[1
]}

Hui, Wun-Chun ^{[1
]}

Cheuk, Yau-Chuk ^{[1
]}

Wong, Margaret Wan-Nar ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Orthopaed & Traumatol, Shatin, Hong Kong, Peoples R China

来源：

WOUND REPAIR AND REGENERATION | 2008年 / 16卷 / 03期

关键词：

D O I：

10.1111/j.1524-475X.2008.00379.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transforming growth factor beta (TGF-beta) plays active roles in tendon healing. However, the differential effects of TGF-beta isoforms on tendon healing have not been investigated. In cultured tendon fibroblasts, we tested the effects of TGF-beta 1, beta 2, and beta 3 on the mRNA levels of COL1A1 and COL3A1 by quantitative real-time polymerase chain reaction. We also investigated the expression of TGF-beta isoforms, TGF-beta receptors, procollagen Type I and Type III in a rat model of tendon healing. We found that TGF-beta 3 exhibited the highest potency in stimulating COL1A1 and COL3A1. TGF-beta 1 exerted antagonistic effects to TGF-beta 2 and beta 3. All TGF-beta isoforms and procollagen Type I were confined to the edges of the healing tendon at day 28 postinjury. Our results indicated that interaction of TGF-beta isoforms exist in the regulation of collagen synthesis in tendon fibroblasts. Their effects may be further complicated by uneven spatial distribution of TGF-beta and TGF-beta receptors in healing tendons.

引用

页码：399 / 407

页数：9

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