Association between Cationic Liposomes and Low Molecular Weight Hyaluronic Acid

被引：33

作者：

Gasperini, Antonio A. M. ^{[1
]}

Puentes-Martinez, Ximena E. ^{[1
,2
]}

Balbino, Tiago Albertini ^{[3
]}

Rigoletto, Thais de Paula ^{[4
]}

Cavalcanti Correa, Gabriela de Sa ^{[3
]}

Cassago, Alexandre ^{[5
]}

Portugal, Rodrigo Villares ^{[5
]}

de La Torre, Lucimara Gaziola ^{[3
]}

Cavalcanti, Leide P. ^{[1
]}

机构：

[1] CNPEM, Brazilian Synchrotron Light Lab, BR-13083970 Campinas, SP, Brazil

[2] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, BR-12231280 Sao Paulo, Brazil

[3] Univ Estadual Campinas, Sch Chem Engn, BR-13083970 Campinas, SP, Brazil

[4] Ctr Univ Fac Associadas Ensino UNIFAE, BR-13870377 Sao Paulo, Brazil

[5] CNPEM, Brazilian Nanotechnol Natl Lab, BR-13083970 Campinas, SP, Brazil

来源：

LANGMUIR | 2015年 / 31卷 / 11期

基金：

巴西圣保罗研究基金会;

关键词：

GENE-THERAPY; DELIVERY; CD44; COMPLEXES; PROSPECTS; PROGRESS; CANCER; CELLS; EPC/DOTAP/DOPE; NANOPARTICLES;

D O I：

10.1021/la5045865

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential.

引用

页码：3308 / 3317

页数：10

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