Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor

被引：94

作者：

Morita, Masatomo ^{[1
]}

Ohnishi, Makoto ^{[1
]}

Arakawa, Eiji ^{[1
]}

Bhuiyan, N. A. ^{[2
]}

Nusrin, Suraia ^{[2
]}

Alam, Munirul ^{[2
]}

Siddique, A. K. ^{[2
]}

Qadri, Firdausi ^{[2
]}

Izumiya, Hidemasa ^{[1
]}

Nair, G. Balakrish ^{[2
]}

Watanabe, Haruo ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Shinjuku Ku, Tokyo 1628640, Japan

[2] Int Ctr Diarrheal Dis Res, Dhaka 1212, Bangladesh

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2008年 / 52卷 / 06期

关键词：

altered El Tor strain; biotype; cholera toxin; Vibrio cholerae O1;

D O I：

10.1111/j.1348-0421.2008.00041.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.

引用

页码：314 / 317

页数：4

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