Automated selection of anti-protein aptamers

被引：286

作者：

Cox, JC

Ellington, AD ^{[1
]}

机构：

[1] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA

[2] Univ Texas, Inst Cellular & Mol Biol, Austin, TX 78712 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2001年 / 9卷 / 10期

关键词：

D O I：

10.1016/S0968-0896(01)00028-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vitro selection of nucleic acid binding species (aptamers) is frequently repetitive, time-consuming, and poorly adapted to high-throughput applications. We have adapted automated workstations to select anti-protein aptamers; as an example, we demonstrated the selection of anti-lysozyme aptamers that function as efficient inhibitors of cell lysis. The increases in throughput brought about by automation should potentiate the application of aptamer technology to the rapidly growing field of proteomics. (C) 2001 Published by Elsevier Science Ltd.

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页码：2525 / 2531

页数：7

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