Tilted Brownian ratchet for DNA analysis

被引：83

作者：

Huang, LR

Cox, EC

Austin, RH ^{[1
]}

Sturm, JC

机构：

[1] Princeton Univ, Dept Phys, Ctr Photon & Optoelect Mat, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Elect Engn, Ctr Photon & Optoelect Mat, Princeton, NJ 08544 USA

[3] Princeton Univ, Dept Mol Biol, Ctr Photon & Optoelect Mat, Princeton, NJ 08544 USA

来源：

ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 24期

关键词：

D O I：

10.1021/ac0348524

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, we report a factor of 3 improvement in the resolution and a factor of 10 improvement in the speed of fractionation of similar to100-kb DNA molecules in Brownian ratchet arrays. In our device, the electrophoretic flow is tilted at a small angle relative to the array axis. Tilting accelerates the fractionation speed because a higher fraction of the diffusing molecules is "ratcheted" at each step in the array. Molecules of lengths 48.5 and 164 kb can be separated in similar to70 min with a resolution of similar to3.8, using a 12-mm-long array. The Brownian ratchet arrays are not limited to DNA separation, but can, in principle, be used for any particle in this size range.

引用

页码：6963 / 6967

页数：5

共 14 条

[1] Thermodynamics and kinetics of a Brownian motor [J].