Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes

被引:189
作者
Agarwal, S [1 ]
Sairam, RK [1 ]
Srivastava, GC [1 ]
Meena, RC [1 ]
机构
[1] Indian Agr Res Inst, Div Plant Physiol, New Delhi 110012, India
关键词
ascorbate peroxidase; catalase; glutathione reductase; hydrogen peroxide; lipid peroxidation; membrane stability index; superoxide dismutase; Triticum aestivum;
D O I
10.1007/s10535-005-0048-z
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Abscisic acid (ABA) and salicylic acid (SA) were sprayed on leaves of wheat genotypes C 306 and Hira at 25 and 40 d after sowing under moderate water stress (-0.8 MPa) imposed by adding PEG-6000 in nutrient solution. ABA and SA increased the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and catalase in comparison to unsprayed control plants. Both ABA and SA treatments decreased the contents of hydrogen peroxide and thiobarbituric acid reactive substances, a measure of lipid peroxidation, compared to unsprayed plants. The beneficial effect of increase in antioxidant enzymes activity and decrease in oxidative stress was reflected in increase in chlorophyll and carotenoid contents, relative water content, membrane stability index, leaf area and total biomass over control plants. The lower concentrations of ABA (0.5 mM) and SA (1.0 mM) were generally more effective than higher concentrations.
引用
收藏
页码:541 / 550
页数:10
相关论文
共 49 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2]  
Alscher RG, 1997, PHYSIOL PLANTARUM, V100, P224, DOI 10.1034/j.1399-3054.1997.1000203.x
[3]   COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].
ARNON, DI .
PLANT PHYSIOLOGY, 1949, 24 (01) :1-15
[4]   SUPEROXIDE-DISMUTASE AND STRESS TOLERANCE [J].
BOWLER, C ;
VANMONTAGU, M ;
INZE, D .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1992, 43 :83-116
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   GENE-EXPRESSION REGULATED BY ABSCISIC-ACID AND ITS RELATION TO STRESS TOLERANCE [J].
CHANDLER, PM ;
ROBERTSON, M .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1994, 45 :113-141
[7]   Physiological and biochemical responses of hexaploid and tetraploid wheat to drought stress [J].
Chandrasekar, V ;
Sairam, RK ;
Srivastava, GC .
JOURNAL OF AGRONOMY AND CROP SCIENCE-ZEITSCHRIFT FUR ACKER UND PFLANZENBAU, 2000, 185 (04) :219-227
[8]   ACTIVE OXYGEN SPECIES IN THE INDUCTION OF PLANT SYSTEMIC ACQUIRED-RESISTANCE BY SALICYLIC-ACID [J].
CHEN, ZX ;
SILVA, H ;
KLESSIG, DF .
SCIENCE, 1993, 262 (5141) :1883-1886
[9]   Changes in the activities of antioxidant enzymes in response to virus infection and hormone treatment [J].
Clarke, SF ;
Guy, PL ;
Burritt, DJ ;
Jameson, PE .
PHYSIOLOGIA PLANTARUM, 2002, 114 (02) :157-164
[10]   2 INDUCERS OF PLANT DEFENSE RESPONSES, 2,6-DICHLOROISONICOTINIC ACID AND SALICYLIC-ACID, INHIBIT CATALASE ACTIVITY IN TOBACCO [J].
CONRATH, U ;
CHEN, ZX ;
RICIGLIANO, JR ;
KLESSIG, DF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7143-7147