Production of a novel medium chain length poly(3-hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold

被引：48

作者：

Basnett, Pooja ^{[1
]}

Lukasiewicz, Barbara ^{[1
]}

Marcello, Elena ^{[1
]}

Gura, Harpreet K. ^{[2
]}

Knowles, Jonathan C. ^{[3
,4
,5
]}

Roy, Ipsita ^{[1
]}

机构：

[1] Univ Westminster, Fac Sci & Technol, London, England

[2] Aarhus Univ, Aarhus, Denmark

[3] UCL, Eastman Dent Inst, London, England

[4] Dankook Univ, Dept Nanobiomed Sci, Cheonan 330714, South Korea

[5] Dankook Univ, Plus NBM Global Res Ctr Regenerat Med BK21, Cheonan 330714, South Korea

来源：

MICROBIAL BIOTECHNOLOGY | 2017年 / 10卷 / 06期

基金：

欧盟地平线“2020”;

关键词：

POLYHYDROXYALKANOATES PHAS PRODUCTION; PSEUDOMONAS-MENDOCINA NK-01; SOLE CARBON SOURCE; PROTEIN ADSORPTION; RALSTONIA-EUTROPHA; ALGINATE OLIGOSACCHARIDES; BIOMEDICAL APPLICATIONS; BIODEGRADABLE POLYMERS; SURFACE-ROUGHNESS; BACTERIAL STRAINS;

D O I：

10.1111/1751-7915.12782

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

This study demonstrated the utilization of unprocessed biodiesel waste as a carbon feedstock for Pseudomonas mendocinaCH50, for the production of PHAs. A PHA yield of 39.5% CDM was obtained using 5% (v/v) biodiesel waste substrate. Chemical analysis confirmed that the polymer produced was poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate) or P(3HHx-3HO-3HD-3HDD). P(3HHx-3HO-3HD-3HDD) was further characterized and evaluated for its use as a tissue engineering scaffold (TES). This study demonstrated that P(3HHx-3HO-3HD-3HDD) was biocompatible with the C2C12 (myoblast) cell line. In fact, the % cell proliferation of C2C12 on the P(3HHx-3HO-3HD-3HDD) scaffold was 72% higher than the standard tissue culture plastic confirming that this novel PHA was indeed a promising new material for soft tissue engineering.

引用

页码：1384 / 1399

页数：16

共 61 条

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