13C-13C NOESY:: A constructive use of 13C-13C spin-diffusion

被引：31

作者：

Bertini, I ^{[1
]}

Felli, IC

Kümmerle, R

Luchinat, C

Pierattelli, R

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy

[2] Univ Florence, Dept Chem, I-50121 Florence, Italy

[3] Bruker BioSpin AG, Fallanden, Switzerland

[4] Univ Florence, Dept Agr Biotechnol, I-50121 Florence, Italy

来源：

JOURNAL OF BIOMOLECULAR NMR | 2004年 / 30卷 / 03期

关键词：

C-13 direct detection; Cu; Zn-SOD; NOESY; spin diffusion; superoxide dismutase;

D O I：

10.1007/s10858-005-1679-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-13-C-13 NOESY experiments were performed under long mixing time conditions on reduced human superoxide dismutase (32 kDa, N-15, C-13 and 70% H-2 labeled). C-13-C-13 couplings were successfully eliminated through post-processing of in-phase-anti-phase (IPAP) data. It appears that at mixing time tau(m) of 3.0 s the spin diffusion mechanism allows the detection of 96% of the two-bond correlations involving C' and C-beta. The interpretation was confirmed by simulations. This approach broadens the range of applicability of C-13-C-13 NOESY spectroscopy.

引用

页码：245 / 251

页数：7

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