Cyro-hydrogel for the construction of a tyrosinase-based biosensor

被引:38
作者
Deng, Q [1 ]
Guo, YZ [1 ]
Dong, SJ [1 ]
机构
[1] CHINESE ACAD SCI,CHANGCHUN INST APPL CHEM,ELECTROANALYT CHEM LAB,CHANGCHUN 130022,PEOPLES R CHINA
基金
中国国家自然科学基金;
关键词
biosensors; cyro-hydrogel; tyrosinase;
D O I
10.1016/0003-2670(95)00498-X
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new immobilization method for construction of a tyrosinase based biosensor is described. A simple physical freezing technique was adopted for preparation. The immobilized enzyme yields specific activities that are more than 22% of the soluble enzyme. The enzyme electrode can be stored in dry state for more than three months without any loss of activity. The biosensor was applied to the determination of several phenols and o-diphenols. The lowest detect limit is 0.02 mu mol/1 and the linear range was 1.0 X 10(-7)-1.0 X 10(-4) mol/1 for catechol. The kinetic parameters have also been calculated.
引用
收藏
页码:71 / 77
页数:7
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