Adventitial expression of recombinant eNOS gene restores NO production in arteries without endothelium

被引:48
作者
Tsutsui, M
Chen, AFY
O'Brien, T
Crotty, TB
Katusic, ZS
机构
[1] Mayo Clin & Mayo Fdn, Dept Anesthesiol, Rochester, MN 55905 USA
[2] Mayo Clin, Dept Pharmacol, Rochester, MN USA
[3] Mayo Clin, Div Endocrinol, Rochester, MN USA
[4] Mayo Clin, Div Metab, Rochester, MN USA
[5] Mayo Clin, Div Metab, Rochester, MN USA
关键词
fibroblasts; adenoviral vector; gene transfer; gene therapy; bradykinin;
D O I
10.1161/01.ATV.18.8.1231
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The current study was designed to determine the effect of recombinant endothelial nitric ic oxide synthase (eNOS) gene expression on endothelium-dependent relaxations to bradykinin in isolated canine basilar, coronary, or femoral arteries. Arterial rings were exposed ex vivo (30 minutes at 37 degrees C) to an adenoviral vector encoding either the eNOS gene (AdCMVeNOS) or the beta-galactosidase reporter gene (AdCMV beta-Gal). Twenty-four hours after transduction, transgene expression was evident mainly in the adventitia. Expression of recombinant proteins was much higher in basilar arteries than in coronary or femoral arteries. Rings of control, AdCMV beta-Gal, and AdCMVeNOS arteries with and without endothelium were suspended for isometric tension recording. Levels of cGMP were measured by radioimmunoassay. In AdCMVeNOS basilar arteries with endothelium, relaxations to low concentrations of bradykinin (3x10(-11) to 10(-9) mol/L) were significantly augmented. In contrast, in coronary and femoral arteries with endothelium, AdCMVeNOS transduction did not affect relaxations to bradykinin. Removal of the endothelium abolished bradykinin-induced relaxations in control and AdCMV beta-Gal basilar arteries. However, in basilar arteries transduced with AdCMVeNOS even when the endothelium was removed, stimulation with bradykinin (3x10(-11) to 10(-9) mol/L) caused relaxations as well as increases in cGMP production. The relaxations to bradykinin were completely blocked by an NOS inhibitor, N-G-nitro-L-arginine methyl ester. Electron microscopic analysis revealed that recombinant eNOS protein was expressed in fibroblasts of the basilar artery adventitia. These results suggest that genetically modified adventitial fibroblasts may restore production of NO in cerebral arteries without endothelium. Our findings support a novel concept in vascular biology that fibroblasts in the adventitia may play a role in the regulation of vascular tone after successful transfer and expression of recombinant eNOS gene.
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页码:1231 / 1241
页数:11
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