Protease inhibitors in buckwheat seeds: Comparison of anionic and cationic inhibitors

被引:21
作者
Dunaevsky, YE
Pavlukova, EB
Beliakova, GA
Tsybina, TA
Gruban, TN
Belozersky, MA
机构
[1] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119899, Russia
[2] AN Bakh Biochem Inst, Moscow 117071, Russia
关键词
Fagopyrum esculentum; buckwheat; protease inhibitors; reactive site; seeds; trypsin;
D O I
10.1016/S0176-1617(98)80032-1
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Protease inhibitors in buckwheat seeds are separated into two main groups - anionic and cationic inhibitors, according to their behaviour on ion-exchange chromatography. Three anionic inhibitors (BWI-1a, BWI-2a and BWI-4a) and two cationic (BWI-2c and BWI-4c) were purified to homogeneity and characterized. Molecular masses of anionic inhibitors were in the range 7.7-9.2 kD and of cationic 6.0 kD. Both anionic and cationic inhibitors were highly pH- and thermostable. All anionic and cationic inhibitors inhibited trypsin. In addition to trypsin BWI-1a and BWI-2a inhibited chymotrypsin, however, less effectively. Cationic inhibitor BWI-4c besides trypsin and chymotrypsin inhibited also bacterial subtilisin. Inhibitors BWI-1a, BWI-2a, BWI-4a and BWI-2c contain an Arg residue at the reactive site whereas BWI-4c contains a Lys residue. According ro determined amino acid sequences anionic inhibitors BWI-1a, BWI-2a and BWI-4a belong to the potato proteinase inhibitor I family.
引用
收藏
页码:696 / 702
页数:7
相关论文
共 27 条
[1]  
BELOZERSKY MA, 1995, FEBS LETT, V371, P264, DOI 10.1016/0014-5793(95)00899-K
[3]   Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant [J].
Duan, XL ;
Li, XG ;
Xue, QZ ;
AboElSaad, M ;
Xu, DP ;
Wu, R .
NATURE BIOTECHNOLOGY, 1996, 14 (04) :494-498
[4]  
Dunaevsky YE, 1996, BIOCHEMISTRY-MOSCOW+, V61, P1350
[5]  
ELPIDINA EN, 1991, PLANTA, V185, P46, DOI 10.1007/BF00194513
[6]   PREPARATION AND PROPERTIES OF 2 NEW CHROMOGENIC SUBSTRATES OF TRYPSIN [J].
ERLANGER, BF ;
COHEN, W ;
KOKOWSKY, N .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 95 (02) :271-&
[7]   ACCUMULATION OF A METALLO-CARBOXYPEPTIDASE INHIBITOR IN LEAVES OF WOUNDED POTATO PLANTS [J].
GRAHAM, JS ;
RYAN, CA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1981, 101 (04) :1164-1170
[8]   DETERMINATION OF FREE AMINO GROUPS IN PROTEINS BY TRINITROBENZENESULFONIC ACID [J].
HABEEB, AFS .
ANALYTICAL BIOCHEMISTRY, 1966, 14 (03) :328-&
[9]   STRUCTURE AND REGULATED EXPRESSION OF KUNITZ CHYMOTRYPSIN INHIBITOR GENES IN WINGED BEAN (PSOPHOCARPUS-TETRAGONOLOBUS (L) DC) [J].
HABU, Y ;
PEYACHOKNAGUL, S ;
UMEMOTO, K ;
SAKATA, Y ;
OHNO, T .
JOURNAL OF BIOCHEMISTRY, 1992, 111 (02) :249-258
[10]   TRYPSIN AND ELASTASE INHIBITORS FROM BITTER GOURD (MOMORDICA-CHARANTIA LINN) SEEDS - PURIFICATION, AMINO-ACID-SEQUENCES, AND INHIBITORY ACTIVITIES OF 4 NEW INHIBITORS [J].
HAMATO, N ;
KOSHIBA, T ;
PHAM, TN ;
TATSUMI, Y ;
NAKAMURA, D ;
TAKANO, R ;
HAYASHI, K ;
HONG, YM ;
HARA, S .
JOURNAL OF BIOCHEMISTRY, 1995, 117 (02) :432-437