Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy

被引：117

作者：

Grigorieff, Nikolaus ^{[1
,4
]}

Harrison, Stephen C. ^{[2
,3
,4
]}

机构：

[1] Brandeis Univ, Rosenstiel Basic Med Sci Res Ctr, Waltham, MA 02154 USA

[2] Harvard Univ, Sch Med, Boston, MA 02115 USA

[3] Childrens Hosp, Boston, MA 02115 USA

[4] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2011年 / 21卷 / 02期

关键词：

SINGLE-PARTICLE RECONSTRUCTIONS; CRYOELECTRON MICROSCOPY; 3-DIMENSIONAL RECONSTRUCTION; ANGSTROM RESOLUTION; RADIATION-DAMAGE; CRYO-EM; IMAGE-RECONSTRUCTION; SPHERICAL VIRUSES; FOURIER SYNTHESIS; PHASE-CONTRAST;

D O I：

10.1016/j.sbi.2011.01.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfil predictions made 15 years ago that single-particle cryo-EM techniques could visualize molecular detail at 3-4 angstrom resolution. This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry.

引用

收藏

页码：265 / 273

页数：9

相关论文

共 73 条

[1] MAGNIFICATION MISMATCHES BETWEEN MICROGRAPHS - CORRECTIVE PROCEDURES AND IMPLICATIONS FOR STRUCTURAL-ANALYSIS [J].

ALDROUBI, A ;

TRUS, BL ;

UNSER, M ;

BOOY, FP ;

STEVEN, AC .

ULTRAMICROSCOPY, 1992, 46 (1-4) :175-188

[2] Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome [J].

Armache, Jean-Paul ;

Jarasch, Alexander ;

Anger, Andreas M. ;

Villa, Elizabeth ;

Becker, Thomas ;

Bhushan, Shashi ;

Jossinet, Fabrice ;

Habeck, Michael ;

Dindar, Guelcin ;

Franckenberg, Sibylle ;

Marquez, Viter ;

Mielke, Thorsten ;

Thomm, Michael ;

Berninghausen, Otto ;

Beatrix, Birgitta ;

Soeding, Johannes ;

Westhof, Eric ;

Wilson, Daniel N. ;

Beckmann, Roland .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (46) :19754-19759

[3] Radiation damage effects at four specimen temperatures from 4 to 100 K [J].

Bammes, Benjamin E. ;

Jakana, Joanita ;

Schmid, Michael F. ;

Chiu, Wah .

JOURNAL OF STRUCTURAL BIOLOGY, 2010, 169 (03) :331-341

[4] Determination of the fold of the core protein of hepatitis B virus ky electron cryomicroscopy [J].

Bottcher, B ;

Wynne, SA ;

Crowther, RA .

NATURE, 1997, 386 (6620) :88-91

[5] USE OF SPOT-SCAN PROCEDURE FOR RECORDING LOW-DOSE MICROGRAPHS OF BEAM-SENSITIVE SPECIMENS [J].

BULLOUGH, P ;

HENDERSON, R .

ULTRAMICROSCOPY, 1987, 21 (03) :223-229

[6] Design of a microfabricated, two-electrode phase-contrast element suitable for electron microscopy [J].

Cambie, Rossana ;

Downing, Kenneth H. ;

Typke, Dieter ;

Glaeser, Robert M. ;

Jin, Jian .

ULTRAMICROSCOPY, 2007, 107 (4-5) :329-339

[7] A dose-rate effect in single-particle electron microscopy [J].

Chen, James Z. ;

Sachse, Carsten ;

Xu, Chen ;

Mielke, Thorsten ;

Spahn, Christian M. T. ;

Grigorieff, Nikolaus .

JOURNAL OF STRUCTURAL BIOLOGY, 2008, 161 (01) :92-100

[8] SIGNATURE: A single-particle selection system for molecular electron microscopy [J].

Chen, James Z. ;

Grigorieff, Nikolaus .

JOURNAL OF STRUCTURAL BIOLOGY, 2007, 157 (01) :168-173

[9] Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM [J].

Chen, James Z. ;

Settembre, Ethan C. ;

Aoki, Scott T. ;

Zhang, Xing ;

Bellamy, A. Richard ;

Dormitzer, Philip R. ;

Harrison, Stephen C. ;

Grigorieff, Nikolaus .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (26) :10644-10648

[10] Structure of small virus-like particles assembled from the L1 protein of human papillomavirus 16 [J].

Chen, XJS ;

Garcea, RL ;

Goldberg, I ;

Casini, G ;

Harrison, SC .

MOLECULAR CELL, 2000, 5 (03) :557-567

← 1 2 3 4 5 6 7 8 →