Procion Green H-E4BD-immobilized porous poly(hydroxyethylmethacrylate) ion-exchange membrane:: preparation and application to lysozyme adsorption

Lysozyme adsorption onto Procion Green HE-4BD-immobilized poly(2-hydroxyethylmethacrylate) (pHEMA) membrane were investigated. The membrane were prepared by ultraviolet-initiated photopolymerization of HEMA in the presence of an initiator (alpha-alpha'-azoisobutyronitrile; AIBN). The water content of the dye-immobilized membrane was 69%, the amount of immobilized dye on the membrane was 0.544 mu mol ml(-1) and it used in the lysozyme adsorption studies. Lysozyme adsorption on these membranes from aqueous solutions containing different amounts of lysozyme at different pH was investigated in batch reactors. Lysozyme adsorption capacity of the dye-immobilized membrane was 13.33 mg ml(-1). The maximum lysozyme adsorption capacity (q(m)) of the dye-immobilized wet membrane was 14.14 mg ml(-1) and the dissociation constant (kd) value was found to be 0.707 mg ml(-1) lysozyme. More than 95% of the adsorbed lysozyme were desorbed in 120 min in the desorption medium containing 0.5 M KCl at pH 6.0. (C) 2001 Elsevier Science B.V. All rights reserved.