Analysis of urinary drugs of abuse by a multianalyte capillary electrophoretic immunoassay

被引:55
作者
Caslavska, J
Allemann, D
Thormann, W
机构
[1] Univ Bern, Dept Clin Pharmacol, CH-3010 Bern, Switzerland
[2] Dept Hlth State Bern, Pharmaceut Control Lab, CH-3001 Bern, Switzerland
关键词
immunoassay; validation; methadone; opiates; benzoylecgonine; amphetamines;
D O I
10.1016/S0021-9673(99)00115-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper characterizes a novel multianalyte competitive binding, electrokinetic capillary-based immunoassay for urinary methadone, opiates, benzoylecgonine (cocaine metabolite) and amphetamines. After incubation of 25 mu l urine with the reactants for several minutes in the presence of an internal standard, a small aliquot of the mixture is applied onto a fused-silica capillary and the unbound fluorescein labelled drug tracers are monitored by capillary electrophoresis with on-column laser induced fluorescence detection. The multianalyte assay is shown to be rapid, simple, quantitative, capable of recognizing urinary drug concentrations greater than or equal to 30 ng/ml and suitable for screening of patient urines. Data are demonstrated to compare well with those obtained by routine screening methods based on enzyme multiplied immunoassay techniques and fluorescence polarization immunoassays. The electrokinetic capillary assay has been validated via analysis of external quality control urines and confirmation analysis of patient urines using GC-MS. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:197 / 211
页数:15
相关论文
共 38 条