Analysis of steroid hormones in effluents of wastewater treatment plants by liquid chromatography-tandem mass spectrometry

被引：112

作者：

Ingrand, V ^{[1
]}

Herry, G ^{[1
]}

Beausse, J ^{[1
]}

de Roubin, MR ^{[1
]}

机构：

[1] Anjou Rech Vivendi Water, Emerging Parameters, F-94417 St Maurice, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1020卷 / 01期

关键词：

water analysis; steroids;

D O I：

10.1016/S0021-9673(03)00770-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper presents the development of an analytical procedure for the determination of two sexual steroid hormones: 17beta-estradiol and estrone, and the synthetic contraceptive estrogen, 17alpha-ethynylestradiol in effluents of wastewater treatment plants. Samples are extracted via solid-phase extraction using C-18 cartridges. Extracts in ethyl acetate are then purified with a liquid-liquid separation with aqueous sodium chloride, then with a clean-up on a Florisil cartridge. Steroids are analyzed using an LC-MS-MS ion trap system. Internal quantification with the corresponding deuterated steroids leads to limits of quantification at 5 ng/l for estrone and 10 ng/l for estradiol and ethynylestradiol. In mineral spiked water, recoveries are 91% for 17beta-estradiol, 97% for estrone and 87% for 17alpha-ethynylestradiol and RSDs are 15% for 17beta-estradiol, 11% for estrone and 23% for 17alpha-ethynylestradiol. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：99 / 104

页数：6

共 12 条

[1] Analysis and occurrence of estrogenic hormones and their glucuronides in surface water and waste water in The Netherlands [J].

Belfroid, AC ;

Van der Horst, A ;

Vethaak, AD ;

Schäfer, AJ ;

Rijs, GBJ ;

Wegener, J ;

Cofino, WP .

SCIENCE OF THE TOTAL ENVIRONMENT, 1999, 225 (1-2) :101-108

[2] Identification of estrogenic chemicals in STW effluent. 1. Chemical fractionation and in vitro biological screening [J].

Desbrow, C ;

Routledge, EJ ;

Brighty, GC ;

Sumpter, JP ;

Waldock, M .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1998, 32 (11) :1549-1558

[3] Environmental endocrine disrupters [J].

Keith, LH .

PURE AND APPLIED CHEMISTRY, 1998, 70 (12) :2319-2326

[4]

KOMORI K, 2001, P INT WAT ASS 2 WORL

[5]

Laganà A, 2000, RAPID COMMUN MASS SP, V14, P401, DOI 10.1002/(SICI)1097-0231(20000331)14:6<401::AID-RCM883>3.0.CO

[6]

2-7

[7]

Lee HB, 1998, J AOAC INT, V81, P1209

[8] Identification of estrogenic chemicals in STW effluent. 2. In vivo responses in trout and roach [J].

Routledge, EJ ;

Sheahan, D ;

Desbrow, C ;

Brighty, GC ;

Waldock, M ;

Sumpter, JP .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1998, 32 (11) :1559-1565

[9] An updated review of environmental estrogen and androgen mimics and antagonists [J].

Sonnenschein, C ;

Soto, AM .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1998, 65 (1-6) :143-150

[10] Xenoendocrine disrupters - environmental impacts [J].

Sumpter, JP .

TOXICOLOGY LETTERS, 1998, 103 :337-342

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